Levels of cellular DNA polymerases in synchronized bovine paravovirus-infected cells.

Effect of Cadmium and Lead on [h]-thymidine Uptake in Sheep Lymphocytes Infected with Bovine Leukaemia Virus

Effect of cadmium (Cd II) and lead (Pb II) was determined on cell proliferation, DNA synthesis, DNA repair, and DNA polymerase activity. The experiments were done on lymphocytes obtained from sheep infected with bovine leukaemia virus (BLV) and non-infected animals. All methods were based on [ H]-thymidine incorporation into DNA and performed in cell cultures stimulated by mitogens. Lymphocytes...

In silico analysis of Ta9 gene polymorphism in an Iranian Theileria annulata schizont-infected cell line S15 vaccine strain and native isolates

Bovine theileriosis is a tick-borne disease caused by obligate intracellular parasites related to the genus Theileria. Cellular immune responses protect cattle against pathogens through the activation of immune cells. Nowadays, live, attenuated vaccine of Theileria annulata (T. annulata) is being produced in Iran and is recommended for active cattle immunization. Detection of the immunogenic  a...

DNA damage in dental pulp mesenchymal stem cells: An in vitro study

The aim of this study was to evaluate the potential use of a DNA comet assay, DNA fragmentation fluorimetric assay and reactive oxygen species levels as potential biomarkers of genome conditions of dental pulp stem cells (DPSCs) isolated from dog canine teeth. Mesenchymal stem cells were isolated from the dental pulp collected from dog teeth. The results obtained suggest the ideal moment for cl...

Inhibition of synchronized cellular deoxyribonucleic acid synthesis during Newcastle disease virus, mengovirus, or reovirus infection.

Cultures of L cells were synchronized with respect to deoxyribonucleic acid (DNA) synthesis with thymidine and 5-fluoro-2'-deoxyuridine (FUdR) and infected with Newcastle disease virus (NDV), mengovirus, or reovirus 3. Inhibition of incorporation of (3)H-cytidine into the DNA of synchronized cells is partially inhibited 2 hr after infection with NDV or mengovirus and nearly completely suppresse...

lsolation of parapox viruses from man and animals: cultivation and cellular changes in bovine fetal spleen cells